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The dilution factor chosen for the series of calibration standards is achievable by using serial dilution. The progression of calibration standard concentration is always a geometric series. Consider the example of making the first standard at 1/3 the concentration of the known, the next calibrant would be 1/9th the concentration of the known and the following two calibrants formed are 1/27th and 1/81st. Dilution factor = mathematical reciprocal of dilution (inverse, exponent always negative) What is the advantages and disadvantages to the serial dilution agar plate procedure Advantage - it has countable viable cells to count. Start studying 13) Serial dilution agar plate. Learn vocabulary, terms, and more with flashcards, games, and other study tools. What is the advantages. As the dilutions progress, you will reach a point where a reasonable number of colonies can be counted and multiplied by the dilution factor to get concentration. Disadvantages- does not separate bacteria like a streak plate and you have to work with liquid agar.